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SDS-PAGE and western blot analysis of five soluble scFvs. (A) The periplasmic expression of five scFv antibodies, including SP164, SP178, SP192, SP218, and SP220, was analyzed by a 12% SDS-PAGE gel. Lane M: Unstained protein marker. (B) A sharp band corresponding to the scFv with a molecular weight of about 27 kDa was observed in western blot analysis. Non-infected Escherichia coli HB2151 was used as the control (HB2151). Lane M: Pre-stained protein marker
