Figure 4.

TRF reduces the iron accumulation in the serum and liver as induced by HFHFD. (a) Serum and liver iron concentrations were determined using a commercial kit to compare the iron overload levels among the four groups. (b) Hepatic mRNA levels for Irp1, Irp2, Tfr1, Tfr2, and Slc40a1 were measured by RT-qPCR, and GAPDH was used as a control. (c) Tfr1 protein levels were measured by western blotting, with GAPDH used as a control. Data are presented as mean ± SEM. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, and ^∗∗∗∗P < 0.0001; ns: not significant.