Fig. 4.

Patient #5, T cell response a Specific CD4 + T cell response measured by flow cytometry. b UMAP with FlowSOM overlay showing the total CD3⁺ T cell compartment of combined samples. 1000 cells were subsetted from every sample. c Heatmap depicting median expression of various markers in FlowSOM-derived clusters shown in (b). d Barplot depicting the frequency of FlowSOM-derived T cell subsets of all CD3⁺ T cells in indicated samples. e Radar chart depicting median expression of indicated markers in FlowSOM-generated T cell subsets. BM samples from patient #5 at initial diagnosis and during WT1 vaccination (day 418) are shown