Effect of GFS on bending/pinching knee joint-evoked responses. (A) Preparation design. Knee joint is clamped by applying a calibrated force with a serrated forceps placed medially-laterally around the knee joint, or is fully bent to evoke the responses. (B) Representative traces showing pinching knee joint-evoked AP firing in a recorded unit before and after GFS. In this bundle, knee-pinching evoked two C-type units pointed by red arrows (CV of 0.95m/s for the big one and 0.74m/s for the small one), which were both suppressed by GFS. Black arrow points to the start of stimulation. (E, F) Effects of 20Hz GFS on AP firing of recorded units in teased L3 dorsal root bundles by pinching (E) and bending (F) the knee joint. (G, H) Effects of 120s-20Hz GFS on AP firing of L4 dorsal root units induced by pinching (G) and bending (H) the knee joint. Data are from n=5 rats with saline injection and n=5 rats with MIA injection. *P<0.05, ** P<0.01, *** P<0.001 by 2-way repeated measure ANOVA followed by Sidak’s post hoc test.