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. Author manuscript; available in PMC: 2023 Nov 1.
Published in final edited form as: Kidney Int. 2022 Jun 26;102(5):1103–1114. doi: 10.1016/j.kint.2022.05.028

Figure 4. CD expression of active BRAF increases ERK phosphorylation and cell proliferation in Pkd1RC/RC kidneys.

Figure 4.

Representative 10-week-old (a) RC/RC and (b) RC/RC; BRafCD kidney sections stained with an antibody to PCNA (red) and DBA (green). There were increased PCNA-positive cells in the cyst-lining epithelia as well as the interstitium (Supplemental Figure S5). Scale bar = 500 μm. (c) Percentage of PCNA-positive nuclei, normalized to total nuclei that were visualized with DAPI (blue). A minimum of 4 × 103 cells per kidney section were counted. P < 0.01, compared to RC/RC (N = 6). (d) Representative immunoblots for P-ERK, ERK, and BRAF in 10-week-old kidneys without (−) and with (+) CD expression of the BRAFV600E transgene. GAPDH was used as a loading control. The number above the blots are P-ERK/ERK and BRAF/GAPDH, respectively. Bar graphs are mean ± SEM for levels of (e) P-ERK/ERK and (f) BRAF/GAPDH for RC/RC and RC/RC; BRafCD kidneys. *P < 0.05, compared to RC/RC (N = 3).