. Author manuscript; available in PMC: 2023 Nov 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Aug 27;1210:123437. doi: 10.1016/j.jchromb.2022.123437

Table 2.

Accuracy and precision of GCDCA-S and CDCA-24G in different matrices.

Drug	Matrix	Level	Nominal Concentratio n (ng/mL)	Inter-Day Precision (CV(%), n=5)	Intra-Day Precision (CV(%), n=20)	Accuracy (Bias(%) , n=20)
GCDCA-S	Mouse Plasma	LLOQ^b	0.500	11.4	9.72	0.270
		LOQ^a	1.50	9.91	7.96	−0.730
		MQC^a	40.0	9.23	8.84	0.0700
		HQC^a	90.0	11.2	9.18	0.960
		Diluted-QC^a	900^*	9.80	8.13	2.62
	Human Plasma	LLOQ^b	0.500	9.03	5.40	−2.28
		LOQ^b	5.00	9.19	5.08	5.96
		MQC^c	400	11.6	6.08	−6.24
		HQC^c	900	7.23	5.84	−8.15
		Diluted-QC^c	9000^*	8.03	6.01	−3.35
CDCA-24G	Mouse Plasma	LLOQ^b	0.500	10.3	7.23	6.60
		LOQ^a	1.50	7.86	5.86	3.03
		MQC^a	40.0	5.63	3.81	4.27
		HQC^a	90.0	6.21	5.16	5.54
		Diluted-QC^a	900^*	4.52	3.40	8.31
	Human Plasma	LLOQ^b	0.500	8.54	5.95	−0.100
		LOQ^b	1.50	7.49	4.71	1.42
		MQC^c	40.0	8.83	6.73	0.0500
		HQC^c	90.0	10.4	7.07	10.9
		Dilution QC^c	900^*	14.7	10.0	10.1

QC samples were prepared using Pooled Male FVB mouse plasma,

QC samples were prepared using water as the matrix,

QC samples were prepared in pooled human K₂-EDTA plasma,

QC samples were measured after 10X of dilution using the respective matrix.