. Author manuscript; available in PMC: 2023 Nov 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Aug 27;1210:123437. doi: 10.1016/j.jchromb.2022.123437

Table 3.

Matrix effect and extraction recovery of GCDCA-S and CDCA in different matrices.

Conc. (ng/mL)	QC Matrices	Matrix Effect		Recovery
Conc. (ng/mL)	QC Matrices	Mean Matrix Effect (%)	CV (%)	Mean Recovery (%)	CV (%)
GCDCA-S
1.5	Pooled mouse plasma	111	10.9	88.8	9.58
90	Pooled mouse plasma	115^a	11.2	120	13.6
5	pooled human plasma	NA^b	NA^b	80.7	1.16
900	pooled human plasma	111	6.70	114	6.72
CDCA-24G
1.5	Pooled mouse plasma	88.0	8.88	89.8	7.26
90	Pooled mouse plasma	109	8.70	104	3.78
1.5	pooled human plasma	NA^b	NA^b	90.6	12.2
90	pooled human plasma	87.1	11.1	94.0	4.91

The calculated mean matrix effect 114.814 was rounded to 115 as 3 significant figures kept.

The matrix effect result of prepared GCDCA-S and CDCA-24G at 5 ng/mL and 1.5 ng/mL in pooled human plasma was not available.