Table 4.
Stability of GCDCA-S and CDCA-24G in different matrices under various conditions.
| Conc. (ng/mL) | QC Matrices | Sample Conditions | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Bench-Top Stabilitya | Long-Term Stabilityb | Re-injection Stabilityc | Freeze-Thaw Stabilityd | ||||||
| Mean Deviation (%) of t = 0 | CV (%) | Mean Deviation (%) of t = 0 | CV (%) | Mean Deviation (%) | CV (%) | Mean Deviation (%) of nominal Conc. | CV (%) | ||
| GCDCA-S | |||||||||
| 1.5 | Pooled mouse plasma | 5.25 | 4.75 | 1.94 | 3.45 | −6.79 | 4.53 | 7.47 | 5.26 |
| 90 | −7.22 | 5.55 | 7.48 | 4.00 | 6.00 | 5.38 | −1.51 | 4.50 | |
| 5 | water | −5.61 | 8.06 | 2.45 | 7.14 | −8.33 | 6.70 | 2.93 | 3.08 |
| 900 | pooled human plasma | −1.54 | 3.68 | 7.75 | 7.23 | 2.16 | 4.59 | −7.09 | 4.66 |
| CDCA-24G | |||||||||
| 1.5 | Pooled mouse plasma | −7.20 | 5.82 | −8.00 | 5.95 | 1.69 | 3.35 | −5.53 | 4.77 |
| 90 | −7.57 | 8.37 | −5.14 | 6.40 | −0.0600 | 7.20 | −14.3 | 9.14 | |
| 1.5 | water | −2.05 | 4.17 | −3.12 | 4.94 | −4.34 | 4.06 | −5.78 | 5.26 |
| 90 | pooled human plasma | −4.68 | 4.00 | 1.94 | 2.63 | −6.50 | 5.85 | −4.45 | 3.96 |
a
Stored at ambient temperature (25 °C) for 6 hours,
b
Stored for 28 days at −80°C,
c
Stored the analyzed samples at auto-sampler (4°C) and reanalyzed samples after 24 hours,
d
After three freeze-thaw cycles.