Extended Data Fig. 8. Effect of macropinocytosis and mitochondria on Col I-controlled PDAC cell growth.
a, Bromodeoxyuridine (BrdU) incorporation into KPC cells plated on ECM mixtures produced by R/R and WT fibroblasts (R:W). Scale bars, 10 μm. b,c, Paren. and IKKαKD KPC (b) or KC (c) cells were plated −/+ WT or R/R ECM and incubated in LG −/+ indicated reagents. Viable cells were measured after 3 days and depicted relative to untreated plastic-plated Paren. cells. (c). IKKα KD efficiency is shown on the right (b). d,e, Viable 1305 (d) or MIA PaCa-2 (e) cells plated as above and incubated in LG −/+ EIPA. f, Viable Paren. and E79Q KPC cells plated, treated, and presented as above. g, Paren. and DDR1KD 1305 or KPC cells plated on indicated ECM preparations and incubated in LG medium −/+ indicated tigecycline concentrations for 24 h. Total viable cells were measured as above and are presented relative to the untreated cells. h, Mitochondrial ATP production calculated from Fig. 6b. i, Liver morphology and tumour numbers (#) 2 wk after i.s. transplantation of Paren. or NHE1KD KPC cells into CCl4-pretreated Col IWT and Col Ir/r mice −/+ EIPA. NHE1 IB is shown on bottom left. ****P < 0.0001. j, H&E and SR staining of s.c. tumours from Fig. 6c. Quantification of the SR positive area is shown on the left. Scale bars, 100 µm. Results in (a) (n = 6 fields), (b–g) (n = 3 independent experiments), (h) (n = 3 per condition), (j) (n = 5 mice) and (i) are mean ± s.e.m. Statistical significance determined by two-tailed t-test. Exact P values in (a−c,f,h) are shown in Source Data.