Extended Data Fig. 10. cCol I stimulates PDAC growth, macropinocytosis and mitochondrial biogenesis through the DDR1–NRF2 axis.

a, IHC of pancreatic sections of CAE-pretreated Col I^WT or Col I^r/r mice 4 wk after orthotopic transplantation of Paren., DDR1^KD and E79Q/DDR1^KD KPC cells. Boxed areas were further magnified. Scale bars, 100 µm. b, Representative images and sizes of s.c. tumours generated by Paren., DDR1^KD (KD) and E79Q/KD 1305 cells transplanted −/+ WT or R/R fibroblasts into Nu/Nu mice. Scale bars, 1 cm. c, Pancreas weight relative to body weight (P/B) of Col I^WT or Col I^r/r mice 4 wk after orthotopic transplantation of Paren., NRF2^KD and TFAM^KD KPC cells. NRF2 and TFAM KD efficiency is shown below. d, H&E staining and CK19 IHC of above pancreata. Image J quantification of tumour area is shown on the left. Scale bars, 100 µm. Results in (b,c) (n = 5 mice), (d) (n = 5 fields) are mean ± s.e.m. Statistical significance was determined by a two-tailed t-test. ****P < 0.0001.

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