Table 2.

Vascular reactivity in vessels isolated from mice ventilated with 21%, 60%, or 100% oxygen and then incubated with 21, 60, or 95 % oxygen during myography. Data reported are model estimates (95% CI) for the maximum relaxation percentage (Emax) and the concentration at which the relaxation percentage is one half of the maximum relaxation percentage (EC50). N represents the number of aortic ring replicates in each experiment. P-values test the null hypothesis that oxygen had no effect on any of the three model parameters using a Wald chi-square test.

Drug	Oxygen (%)	Emax (% relaxation)	EC50 (log molar)	P-value
Acetylcholine (n = 20)	21	76.4 (69.6 to 83.3)*†	−7.59 (−7.72 to −7.47)	<0.001
	60	53.5 (46.7 to 60.3)	−7.62 (−7.76 to −7.48)
	100	53.1 (46.3 to 60.0)	−7.63 (−7.77 to −7.50)
Sodium nitroprusside (n = 20)	21	133.1 (122.9 to 143.3)†	−7.43 (−7.57 to −7.29)*	<0.001
	60	128.3 (118.1 to 138.6)	−7.04 (−7.19 to −6.90)
	100	114.8 (104.6 to 125.0)	−7.31 (−7.45 to −7.16)*
Cinaciguat (n = 12)	21	76.4 (68.8 to 84.0)	−18.1 (−18.3 to −17.9)	0.50
	60	79.1 (71.5 to 86.7)	−18.0 (−18.2 to −17.7)
	100	78.0 (70.4 to 85.6)	−18.2 (−18.5 to −18.0)

^*indicates p<0.05 vs. 60% oxygen, and

^†indicates p<0.05 vs. 100% oxygen for comparisons of specific parameters between individual treatment groups.