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. Author manuscript; available in PMC: 2023 Oct 19.
Published in final edited form as: J Am Chem Soc. 2022 Oct 6;144(41):18861–18875. doi: 10.1021/jacs.2c05030

Figure 9. HDAC10 inhibition and knockdown affect polyamine acetylation.

Figure 9.

Targeted metabolomics quantification by LC-MS/MS of N1-acetylspermidine, N8-acetylspermidine and N1,8-diacetylspermidine in BE(2)-C cells after 24 h treatment with DKFZ-748 or 49 (A) and after knockdown (B). Results in (A) were reproduced in a second, independent experiment (Figure S6). Significance of inhibitor treatment was calculated between DMSO controls and samples by one-way ANOVA, following the Dunnett test for multiple comparison. Significance of knockdown vs. control was determined by unpaired t-test with Welch’s correction. *: p-value < 0.05, ***: <0.001.