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. Author manuscript; available in PMC: 2023 Nov 1.
Published in final edited form as: J Immunol. 2022 Sep 19;209(9):1691–1702. doi: 10.4049/jimmunol.2200320

Figure 4. Function of CD8 and CD4 T cell response to LCMV infections.

Figure 4.

(A) Splenocytes from C57Bl/6 mice infected 30 days prior with the 3 strains of LCMV were ex vivo stimulated with gp33–41 peptide. Representative flow plots depicting the frequency of indicated cytokines and degranulation marker (CD107a) positive cells are shown. Plots are gated on live CD8α+ cells. (B) Kinetics of IFN-γ+ CD8 T cells in spleen after gp33–41 peptide stimulation. (C) Geometric mean fluorescence intensity (gMFI) of IFN-γ expression on responding cells from spleen at day 30 post indicated viral infection. (D) Frequency of IL-2 secreting CD8 T cells among splenocytes at day 30 post indicated viral infection. (E) Ratio of splenic IFN-γ and TNF-α producing cells to gp33 tetramer+ CD8 T cells at day 8 and day 30 post indicated viral infection. (F) Splenocytes from C57Bl/6 mice infected 8- or 31-days prior with the 3 strains of LCMV were ex vivo stimulated with LCMV gp66–77 peptide. Percentage of CD4 T cells producing IFN-γ, IL-2 and TNF-α is shown. Data are representative of two separate experiments with n=3 mice/group per experiment per group. Bars indicate mean ± SEM. *p <0.05, **p <0.001, ***p <0.0001, ns-not significant. Kruskal Wallis one-way ANOVA with Dunn’s multiple comparison test.