Figure 2. CD4⁺ T cells are important for the maintenance of CD8⁺ T_RM following immunization with AdNP.

(A) Experimental design. (B) Number of FluNP-specific CD8⁺ T_RM in the spleen, lung, and bronchoalveolar lavage (BAL) following depletion of CD4⁺ T cells. For isotype control, n = 9 mice total, 2 independent experiments. For anti-CD4, n = 10 mice total, 2 independent experiments. (C) Example staining for CD69 and CD103 subsets amongst FluNP-specific CD8⁺ T_RM in the lung and BAL. (D) Number of CD69⁺ CD103⁺ FluNP-specific CD8⁺ T_RM in the lung and BAL following depletion of CD4⁺ T cells. n = 4–5 mice per group, 2 independent experiments. Significance was determined using a Mann-Whitney test. Data represent mean ± SEM. P values are as follows: * = p<0.05, ** = p<0.01, *** = p<0.001, ns= not significant.