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. Author manuscript; available in PMC: 2023 Nov 1.
Published in final edited form as: J Immunol. 2022 Sep 26;209(9):1778–1787. doi: 10.4049/jimmunol.2200082

Figure 3. Alveolar macrophages are the cell source of persistent influenza NP antigen in AdNP-immunized mice.

Figure 3.

(A) Example gating strategy for alveolar macrophages and dendritic cells in the lung of Ai14 reporter mice immunized with Ad-Cre. (B) Expression of tdTomato by alveolar macrophages (top row) and dendritic cells (bottom row) from mice immunized with either PBS (naïve) or Ad-Cre at indicated timepoints. (C) Frequency of tdTomato+ alveolar macrophages at indicated timepoints. n = 3–5 mice per timepoint, 2 experiments per timepoint. (D) H&E staining and immunofluorescence microscopy of BAL samples from mice that were naïve (top row) or 90 days post-immunization with AdNP (bottom row) showing CD11c (red), influenza nucleoprotein (FluNP, green), and DAPI (blue). Original images were taken at 100X magnification.