Figure 5. Subsequent respiratory viral infections impact the maintenance of influenza NP-specific CD8⁺ T_RM in AdNP-immunized mice.

(A) Experimental design. (B) Number of influenza NP-specific CD8⁺ T_RM in the spleen, lung, and BAL of AdNP-immunized mice subsequently infected with Sendai parainfluenza or mock infected with PBS. n = 5 mice per group, 2 independent experiments. (C) Number of influenza NP-specific CD8⁺ T_RM in the spleen, lung, and BAL of AdNP-immunized mice subsequently infected with Sendai parainfluenza followed by x31 NP⁻ influenza. n = 5–8 mice per group, 2 independent experiments. (D) Number of CD69⁺ CD103⁺ influenza NP-specific CD8⁺ T_RM in the lung and BAL following Sendai parainfluenza infection of AdNP-immunized mice. (E) Number of CD69⁺ CD103⁺ influenza NP-specific CD8⁺ T_RM in the lung and BAL following infection of AdNP-immunized mice with both Sendai parainfluenza and x31 NP⁻ influenza. (F) Frequency of tdTomato⁺ alveolar macrophages in Ai14 reporter mice immunized with Ad-Cre and then either mock infected (PBS) or infected with Sendai parainfluenza and x31 NP⁻ influenza as described in part A. n = 8–14 mice per group, 2 independent experiments. Significance was determined using a Mann-Whitney test. Data represent mean ± SEM. P values are as follows: * = p<0.05, ** = p<0.01, *** = p<0.001, ns= not significant.