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. 2022 Oct 23;13:6308. doi: 10.1038/s41467-022-34064-4

Fig. 2. Doxorubicin-induced CD47 upregulation in osteosarcoma is dependent on the activation of tumor-associated macrophages.

Fig. 2

a–e Flow cytometric analysis of MHC-II (a), iNOS (b), CD86 (c), Arginase 1 (d), and CD206 (e) expression in CD45+ CD11b+ F4/80+ macrophages in HOS tumors on day 22 harvested from mice that received intravenous injection of doxorubicin (DOX) every 2 days (n = 4 mice per group). f Quantitative real-time PCR analysis of the indicated gene expression in HOS tumors treated as described in (a–e) on day 22 (n = 3 mice per group). g Immunohistochemical staining with antibodies as indicated in serial sections of HOS tumors treated with saline or doxorubicin (DOX), in the presence of clodronate (Clod) liposome or phosphate Buffer Saline (PBS) liposome on day 22. Scale bar, 800 μm and 80 μm, respectively. Representative IHC images are shown on the left. Quantitative analyses of IHC staining are shown on the right (n = 4 mice per group). Data are shown as the mean ± SD. ns not significant. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired two-tailed Student t test (a–f) or one-way ANOVA (g). The experiment was performed three times with similar results (a–g). See the Source Data file for the exact P values. Source data are provided as a Source Data file.