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. 2022 Oct 23;13:6308. doi: 10.1038/s41467-022-34064-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Uniform Manifold Approximation and Projection (UMAP) plot showing clusters of seven main cell types (n = 11 osteosarcoma samples of patients). MSC, mesenchymal stem cell. b UMAP plot showing expression profiles of the IL18 gene (n = 11 osteosarcoma samples of patients). c Scatter plots showing the correlation between IL18 and M1 macrophage marker (CD80 and CD86) gene expression in osteosarcoma patients from TARGET (n = 86 patients). Log₂ (TPM + 1) values were used for calculation and visualization. d Scatter plots showing the correlation between IL18 gene expression and M1 macrophage fraction of immune cells in osteosarcoma patients from TARGET (n = 69 patients). M1 macrophage fraction was calculated by CIBERSORT. e Scatter plots showing the correlation between IL18 and CD47 gene expression in osteosarcoma patients from GSE21257 (left; n = 53 patients), GSE33382 (middle; n = 84 patients), and GSE30699 (right; n = 76 patients). f Representative immunohistochemical images showing CD14, IL-18, and CD47 expression in pre- and post-chemotherapy specimens from the same patient. Scale bar, 200 μm. Comparative analyses of immunohistochemistry-based quantification of CD14 and IL-18 expression are shown on the right. 81 pairs of matched specimens were analyzed (n = 81 patients). g Scatter plots showing the correlation between IL-18 and CD47 expression in osteosarcoma specimens before (left) or after (right) chemotherapy. h Scatter plots showing the correlation between changes of IL-18 and CD47 expression during chemotherapy. ΔIL-18/ΔCD47 H-score for each patient was obtained from subtracting IL-18/CD47 H-score of pre-chemotherapy specimen from that of paired post-chemotherapy specimen. Data are shown as the mean ± SD. Pearson correlation test (c–e, g, h) or paired two-tailed Student t test (f). Source data are provided as a Source Data file.