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. 2022 Oct 23;13:6312. doi: 10.1038/s41467-022-34076-0

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© The Author(s) 2022

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Fig. 3 — a 3 kbp SHARP amplicon. b 6 kbp SHARP amplicon. c 200 bp SHAR amplicon using λDNA as a template. d SHARP doubling time estimate for 200 bp product. e Detection limit versus the amplicon length. The area above the detection limit is shaded in gray and labeled detectable. f Doubling time shows that short amplicons replicate at the highest rate. The standard deviation of the copy number in panels a and b is 4%, while for panel c, it is approximated as $3.8 % + 1 / \sqrt{c o p y}$ , see Methods for details. Source data are provided as a Source Data file.