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. 2022 Oct 23;13:6312. doi: 10.1038/s41467-022-34076-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Transforming SHARP-made plasmid to E. coli. b SHARP amplifies sequence prone to forming secondary structure (CAG repeats) at different SSB concentrations. c SHARP kinetics curves at different temperatures, inset shows detection times. d SHARP product at different temperatures. e SHARP at 37 °C with Bsu and Klenow Exo^- DNAPs at variable SSB concentration. The standard deviation of the template copy number and SSB concentration is 4%, see Methods for details. Source data are provided as a Source Data file.