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. 2022 Oct 23;13:6312. doi: 10.1038/s41467-022-34076-0

Table 2.

Premixed SHARP components for amplification of genomic DNA

RxnMix (2X): μL EnzMix (2X): μL
10X Buffer 4 SSB (9 mg mL−1) 0.8
dNTPs (10 mM each) 2 Bst-LF (1.5 mg mL−1) 0.2
ATP (100 mM) 2 PcrA M6 (0.2 mg mL−1) 2
DTT (100 mM) 4 Nuclease free water 1
Total 12 Total 4