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. Author manuscript; available in PMC: 2023 Oct 20.
Published in final edited form as: Cell Chem Biol. 2022 Oct 6;29(10):1517–1531.e7. doi: 10.1016/j.chembiol.2022.09.002

Figure 5. TERT inhibition targets the non-homologous end-joining DSB repair pathway.

Figure 5

(A) Diagram of the Traffic Light repair reporter system. Repair of an I-Sce-induced DSB in individual cells by end-joining (NHEJ) versus homologous recombination (HR) results in expression of mCherry or GFP, detected by flow cytometry.

(B and C) The ratio of NHEJ to HR (left) and the quantification of mCherry+ or GFP+ cells among single cell population (right) in MCF7 (B) and 293T cells (C). Data from three replicates, mean ± SD.

*** P < 0.001; ** 0.001 < P < 0.01; * 0.01< P < 0.05 compared to DMSO treatment (unpaired t-test).