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. Author manuscript; available in PMC: 2023 Oct 20.
Published in final edited form as: Cell Chem Biol. 2022 Oct 6;29(10):1517–1531.e7. doi: 10.1016/j.chembiol.2022.09.002

Figure 7. TERT inhibition and irradiation induces immunogenic senescent CT26 cells capable of stimulating DC function via STING signaling.

Figure 7

(A) Experimental schema for forming and stimulating BMDCs with CT26 cells and assaying APC function.

(B) Quantitative analysis of DC activation/maturation. CT26 cells treated with DMSO, NU-1, or MST-312 ± IR, incubated 5 days in culture, and combined with BMDCs overnight. Data obtained from live CD11c+/CD103+ DC population in triplicates, mean ± SD.

(C and D) Proliferative rate of live CD8+/CD4− (C) and CD8−/CD4+ (D) T cell population. CFSE labeled murine splenocytes were cocultured for 5 days with DCs pre-stimulated by CT26 cells treated with DMSO, NU-1, or MST ± IR.

(E) CT26 cells treated with indicated compounds ± 10 Gy IR and cocultured with BMDCs as in B. Data from three experiments, mean ± SD. MFI, mean fluorescence intensity.

*** P < 0.001, ** 0.001 < P < 0.01, n.s. P > 0.05 (unpaired t-test).

See also Figures S7.