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Antibodies
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| Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 |
Millipore |
Cat#05-636, RRID:AB_309864 |
| 53BP1 Antibody |
Novus |
Cat#NB100-304, RRID:AB_10003037 |
| Brilliant Violet 421™ anti-mouse CD11c Antibody |
BioLegend |
Cat#117329, RRID:AB_10897814 |
| Brilliant Violet 711™ anti-mouse CD103 Antibody |
BioLegend |
Cat#121435, RRID:AB_2686970 |
| APC anti-mouse CD80 Antibody |
BioLegend |
Cat#104713, RRID:AB_313134 |
| PE/Cyanine7 anti-mouse CD86 Antibody |
BioLegend |
Cat#105013, RRID:AB_439782 |
| Alexa Fluor® 488 anti-mouse H-2Kd Antibody |
BioLegend |
Cat#116609, RRID:AB_493066 |
| Purified Rat Anti-Mouse CD45 |
BD Biosciences |
Cat#550539, RRID:AB_2174426 |
| CD11c (D1V9Y) Rabbit mAb |
Cell Signaling Technology |
Cat#97585, RRID:AB_2800282 |
| Granzyme B (E5V2L) Rabbit mAb (Mouse Specific) |
Cell Signaling Technology |
Cat#44153, RRID:AB_2857976 |
| CD8a Monoclonal Antibody (4SM15) |
Thermo Fisher Scientific |
Cat#14-0808-80, RRID:AB_2572860 |
| Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb |
Cell Signaling Technology |
Cat#9718, RRID:AB_2118009 |
| Anti-Ki67 antibody (ab15580) |
Abcam |
Cat#ab15580, RRID:AB_443209 |
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Bacterial and virus strains
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| Lentivirus |
This paper |
NA |
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Biological samples
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Chemicals, peptides, and recombinant proteins
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| NU-1 |
(Betori et al., 2020) |
NA |
| Chrolactomycin |
(Iorio et al., 2012) |
A kind gift of M. Iorio at NAICONS |
| BIBR 1532 |
Cayman Chemical |
Cat#16608 |
| MST-312 |
Cayman Chemical |
Cat#24301 |
| C-178 |
Selleck Chemicals |
Cat#25860 |
| Irinotecan |
Selleck Chemicals |
Cat#S1198 |
| Doxorubicin |
Selleck Chemicals |
Cat#E2516 |
| Etoposide |
Selleck Chemicals |
Cat#S1225 |
| Paclitaxel |
Selleck Chemicals |
Cat#S1150 |
| Alexa488-TelG probe |
PNA Bio |
Cat#F1008 |
| Recombinant Murine GM-CSF |
PeproTech |
Cat#315-03 |
| Recombinant Murine Flt3-Ligand |
PeproTech |
Cat#250-31L |
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Critical commercial assays
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| CometAssay Single Cell Gel Electrophoresis Assay |
R&D systems |
Cat#4250-050-K |
| CellTiter-Glo® 2.0 Cell Viability Assay |
Promega |
Cat#G9242 |
| CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry |
Thermo Fisher Scientific |
Cat#C34554
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Deposited data
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| RNA sequencing data |
This paper |
SRA: PRJNA663346 |
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Experimental models: Cell lines
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| Human: MCF7 |
ATCC |
Cat#HTB-22 |
| Human: MCF7 Tet on |
Takara |
Cat#632108 |
| Human: Saos-2 |
ATCC |
Cat#HTB-85 |
| Human: A549 |
ATCC |
Cat#CCL-185 |
| Human: 293T |
Takara |
Cat# 632180 |
| Mouse: CT26.WT |
ATCC |
Cat#CRL-2638 |
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Experimental models: Organisms/strains
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| Mouse: BALB/cAnNHsd |
Envigo |
Cat#047 |
| Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl /SzJ (NOD scid gamma (NSG)) |
The Jackson Laboratory (Breeding in house) |
JAX:005557 |
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Oligonucleotides
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| NA |
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Recombinant DNA
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| pCVL SFFV-EF1s HA.NLS.Sce |
(Certo et al., 2011) |
Addgene Plasmid #31479 |
| pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro |
(Certo et al., 2011) |
Addgene Plasmid #31482 |
| pCVL SFFV d14GFP Donor |
(Certo et al., 2011) |
Addgene Plasmid #31475 |
| mVenus-hGeminin (1/110)/pCSII-EF-MCS |
(Sakaue-Sawano et al., 2008) |
A kind gift of Atsushi Miyawaki at RIKEN Center for Brain Science, Japan |
| mCherry-hCdt1 (30/120)/pCSII-EF-MCS |
(Sakaue-Sawano et al., 2008) |
A kind gift of Atsushi Miyawaki at RIKEN Center for Brain Science, Japan |
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Software and algorithms
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| Fiji |
(Schindelin et al., 2012) |
https://imagej.github.io/software/fiji/#publication
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| ImageJ comet assay macro |
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https://www.med.unc.edu/microscopy/resources/imagej-plugins-and-macros/comet-assay/
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| GraphPad Prism |
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https://www.graphpad.com/
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| FlowJo |
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https://www.flowjo.com/
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| Olympus OlyVIA software |
https://www.olympus-lifescience.com/en/discovery/image-sharing-made-easy-meet-olyvia/
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https://www.olympus-lifescience.com/en/support/downloads/
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| IncuCyteS3 2019B |
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https://www.sartorius.com/en/products/live-cell-imaging-analysis/live-cell-analysis-software/incucyte-software-v2019b
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| CompuSyn |
(Chou and Martin, 2005) |
https://www.combosyn.com/
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| Reactome |
(Fabregat et al., 2018) |
https://reactome.org/
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| DAVID |
(Huang da et al., 2009) |
https://david.ncifcrf.gov/
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| Ingenuity Pathway Analysis |
(Krämer et al., 2014) |
https://digitalinsights.qiagen.com/products-overview/discovery-insights-portfolio/analysis-and-visualization/qiagen-ipa/
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Other
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| NA |
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