Fig. 10.

ASC-Exos alleviate MSCs senescence and osteoarthritis. (A) Schematic diagram of ASC-Exos restoring stem cell senescence in vitro and alleviating OA progression in vivo. (B) hMSCs were treated with vector (Veh) or exosome (Exo) for SA-β-Gal staining (scale, 50 μm), and quantification. Western blot and quantification of P16 and P21 expression. Heat map of relative mRNA expression levels of SASP-related genes (n = 3). (C) Bone mineral density analysis of joints of OA mice treated with Veh or Exo (n = 15). (D) Saffron solid green staining (scale, 200 μm) and OARSI grade of articular cartilages (n = 15). (E) Immunohistochemical staining (scale, 60 μm) of Ki67 and P16 and quantitative analysis of articular cartilage in OA mice treated with Veh or Exo (n = 15). (F) Venn diagram showing the number of differentially expressed genes in OA mice after Exo treatment. Reproduced under the terms of the CC-BY 4.0 [26]. Copyright 2022, The Authors, published by Springer Nature.