Chemicals, peptides, and recombinant proteins |
|
Sodium deoxycholate (SDC) |
Sigma |
Cat#30970 |
Tris (2-carboxyethyl) phosphine |
Sigma |
Cat#C4706 |
Chloroacetamide |
Sigma |
Cat#C0267 |
LysC |
Wako |
Cat#4548995075888 |
Trypsin |
Promega |
Cat#V5111 |
Acetonitrile |
Sigma |
Cat#75-05-8 |
25% LC-MS grade ammonia |
Fisher chemical |
Cat#533003 |
Trifluoroacetic acid |
Sigma |
Cat#76-05-1 |
Water, Optima L/MS Grade |
Sigma |
Cat#76-05-1 |
|
Deposited data |
|
Proteomics Identifications Database (PRIDE) |
|
PXD027597 |
Full analysis pipeline |
https://github.com/klevdiamanti/multi-tissue_ms_proteomics |
|
|
Software and algorithms |
|
Cytoscape v3.7 |
Shannon et al., 2003 |
|
EnrichmentMap v3.3 |
Merico et al., 2010 |
|
AutoAnnotate v1.3.3 |
Kucera et al., 2016 |
|
limma v3.42.2 |
Ritchie et al., 2015 |
|
Camera |
Wu et al., 2012 |
|
fgsea v1.12.0 |
Korotkevich et al., 2019 |
|
MaxQuant v1.6.0.1 |
Cox and Mann 2008 |
|
Andromeda |
Cox et al., 2011 |
|
Spectronaut v13 |
Bruderer et al., 2015 |
|
|
Other |
|
MSigDB v7.2 (19 September 2020) |
Liberzon et al., 2011 |
|
UniProt |
The UniProt Consortium, 2021 |
|
GO (from MSigDB v7.2) |
Subramanian et al., 2005 |
|
GOslim (02 May 2020) |
Mi et al., 2019 |
|
KEGG (from MSigDB v7.2) |
Kanehisa et al., 2017 |
|
Reactome (from MSigDB v7.2) |
Jassal et al., 2020 |
|
Empore SPE SDB-RPS disc |
Sigma |
Cat#66886-U |
Reprosil-Pur Basic C18, 1.9μm |
Dr. Maisch Gmbh |
Cat#r119.b9 |
EASY-Nlc 1200 system |
Thermo Fisher |
Cat#LC140 |
Q Exactive™ HF-X Hybrid Quadrupole-Orbitrap™ Mass Spectrometer |
Thermo Fisher |
Cat#IQLAAEGAAPFALGMBFZ |