Table 2.
Sequence of primers for quantitative real-time PCR.
| Genes | Nucleotide sequence of primers (5′-3′) | Accession no. | Length, bp |
|---|---|---|---|
| Glucose metabolism | |||
| GLUT1 | F: GCAGGAGATGAAGGAGGAGAGC | XM_021096908.1 | 258 |
| R: ACGAACAGCGACACGACAGT | |||
| GLUT3 | F: GCCCTGAAAGTCCTCGGTTCCT | XM_021092392.1 | 252 |
| R: ACACGGCGTTGATGCCAGAGA | |||
| OGT | F: TTGACTGCCCCTCTATGCTG | NM_001039748.2 | 131 |
| R: CCTGGGATTTCATTTCTGCTG | |||
| GSK3B | F: AGGTCCTAGGGACACCAACA | XM_021068187.1 | 161 |
| R: TGTGTACTCCAACAGACGGC | |||
| G6PC | F: AAGCCAAGCGAAGGTGTGAGC | NM_001113445.1 | 165 |
| R: GGAACGGGAACCACTTGCTGAG | |||
| GR | F: CCAAACTCTGCCTTGTGTGTTC | XM_021080412.1 | 108 |
| R: TGTGCTGTCCTTCCACTGCT | |||
| FOXO1 | F: GCAAATCGAGTTACGGAGGC | NM_214014.3 | 95 |
| R: AATGTCATTATGGGGAGGAGAGT | |||
| Lipid metabolism | |||
| FABPpm | F: ATGGGCTTATACGGTGAGCG | NM_213928.1 | 127 |
| R: CGTTGACAGGAGGGTTGGAA | |||
| FAT/CD36 | F: CTGGTGCTGTCATTGGAGCAGT | XM_021102279.1 | 161 |
| R: CTGTCTGTAAACTTCCGTGCCTGTT | |||
| FBP1 | F: TCCACCGCACGCTGGTCTAT | NM_213979.1 | 129 |
| R: CCAGTCCTCCTGCCTTCTCCAT | |||
| FATP4 | F: AGACACACGTTGGACCTTCC | XM_013993903.2 | 188 |
| R: GCAGGTTGGTGTTGATGAGC | |||
| FATP3 | F: CAGCATAGGGTGACGGTGTT | XM_021089805.1 | 194 |
| R: CCCTCTGTCAGTCCGTAGGT | |||
| CYP7a1 | F: ACCTGACCAGTTCCGAGATG | XM_013996745.2 | 200 |
| R: TATAGGGCACGATGCACAGA | |||
| FADS2 | F: ACGGCCTTCATCCTTGCTAC | NM_001171750.1 | 144 |
| R: GTTGGCAGAGGCACCCTTTA | |||
| SREBP-1c | F: GACCGGCTCTCCATAGACAA | XM_021066226.1 | 229 |
| R: CCTCTGTCTCTCCTGCAACC | |||
| ELOVL5 | F: TACCACCATGCCACTATGCT | XM_021098832.1 | 102 |
| R: GACGTGGATGAAGCTGTTGA | |||
| ELOVL2 | F: ATTCTTCACCACCAGCGAGG | XM_013977421.1 | 131 |
| R: TGCCTGGCTGTTATCACTCG | |||
| Amino acid metabolism | |||
| LAT1 | F: TTTGTTATGCGGAACTGG | XM_013978228.2 | 155 |
| R: AAAGGTGATGGCAATGAC | |||
| CAT-1 | F: TGCCCATACTTCCCGTCC | NM_001012613.1 | 192 |
| R: GGTCCAGGTTACCGTCAG | |||
| PAT1 | F: TGTGGACTTCTTCCTGATTGTC | XM_021077073.1 | 125 |
| R: CATTGTTGTGGCAGTTATTGGT | |||
| EAAT1 | F: GATGGGACCGCCCTCTAT | XM_021076550.1 | 105 |
| R: CGTGGCTGTGATGCTGATG | |||
| EAAT2 | F: GGCTGCTGGACAGGATGA | XM_021085278.1 | 154 |
| R: TAAATGGACTGGGTCTTGGT | |||
| ASCT2 | F: GATTGTGGAGATGGAGGATGTGG | XM_003355984.4 | 127 |
| R: GCGAGTGAAGAGGAAGTAGATGA | |||
| SNAT1 | F: AAGAACCTGGGCTATCTCGG | XM_003355629.4 | 138 |
| R: TGTTGCGTTAGGACTCGTTG | |||
| SNAT4 | F: GCTGTGGCAATCCTGTCACT | XM_021092582.1 | 105 |
| R: CCATCCAAATGCTTTTTCACCCA | |||
| GLUD1 | F: GGAGGCTGACTGTGACATACTGATTC | NM_001244501.2 | 81 |
| R: TTGGCTTTGACTCTAGGTGCATTGG | |||
| GAD1 | F: GGAAGAGAAGAGCAGGCTTGTGAG | XM_005671944.3 | 132 |
| R: CGAGCGAACAGGTTGGAGAAGTC | |||
| OGDH | F: AGACCAGCAGCAGCCAGGAC | XM_021079066.1 | 113 |
| R: GCGTAGTACATCTCCTCCACATAGTTG | |||
| GSTA1 | F: CATCGCCACCAAGTACAACCTCTAC | NM_214389.2 | 91 |
| R: TTCACCCAAATCTGCCACACCTTC | |||
| β-actin | F: CGTTGGCTGGTTGAGAATC | XM_003357928.4 | 132 |
| R: CGGCAAGACAGAAATGACAA | |||
GLUT1 = glucose transporter-1; GLUT3 = glucose transporter-3; OGT = O-linked N-acetylglucosamine transferase; GSK3B = glycogen synthase kinase 3 beta; G6PC = glucose-6-phosphatase catalytic subunit 1; GR = glucocorticoid receptor; FOXO1 = forkhead box O1; FABPpm = plasma membrane fatty acid binding protein; FAT/CD36 = fatty acid transporter/CD36; FABP1 = fatty acid binding protein 1; FATP3 = fatty acid transport protein 3; FATP4 = fatty acid transport protein 4; CYP7a1 = cholesterol-7a-hydroxylase; FADS2 = fatty acid desaturase 2; SREBP-1c = sterol regulatory element binding transcription factor 1c; ELOVL5 = fatty acid elongase 5; ELOVL2 = fatty acid elongase 2; LAT1 = neutral AA transporter 1; CAT-1 = cationic amino acid transporter 1; PAT1 = proton-coupled amino acid transporter 1; EAAT1 = excitatory amino acid transporters 1; EAAT2 = excitatory amino acid transporters 2; ASCT2 = amino-acid transporter 2; SNAT1 = sodium-dependent neutral amino acid transporter 1; SNAT4 = sodium-dependent neutral amino acid transporter 4; GLUD1 = glutamate dehydrogenase 1; GAD1 = glutamate decarboxylase 1; OGDH = recombinant oxoglutarate dehydrogenase; GSTA1 = glutathione S-transferase alpha 1.