TABLE 8.
AMLI reference serum panel
| Specimen | Major antibody | WB band(s) observed | Other antibody(ies) present (by EIA) |
|---|---|---|---|
| A | Centromere | 80-kDa CENP-B, 18-kDa CENP-A (minor) | None |
| B | Scl-70 | 100-kDa Scl-70, 70-kDa Scl-70 (breakdown product) | None |
| D | U1-RNP | 70-kDa U1-RNP, 20 kDa U1-RNP-C | SS-A (contaminant) |
| E | SS-A | 60-kDa SS-A, 52-kDa SS-A | None |
| F | Jo-1 | 55-kDa Jo-1 | SS-A, RNP?a |
| G | SS-B | 52-kDa SS-B, 48-kDa SS-B (breakdown product) | SS-A, U1-RNP? |
| I | Sm | 29- and 28-kDa Sm-B, 14-kDa Sm-E and Sm-F, 70-kDa U1-RNP, 20-kDa U1-RNP-C | dsDNA, U1-RNP, Scl-70? |
| J | dsDNA | 29- and 28-kDa Sm-B, 14-kDa Sm-E Sm-F, 70-kDa U1-RNP, 20-kDa U1-RNP-C | SS-A, U1-RNP?, Scl-70? |
| K | None | None | None |
| L | None | None | None |
a
?, inconclusive.