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. 2022 Sep 26;3(10):100780. doi: 10.1016/j.xcrm.2022.100780
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Horse anti-mouse HRP Cell Signaling Technology Cat# 7076S RRID:AB_330924
Mouse anti-myc mAb Cell Signaling Technology Cat# 2276S RRID:AB_331783
Goat anti-mouse IgG+IgM Jackson ImmunoResearch Cat# 115-005-068 RRID:AB_2338453
Mouse IgG1 lambda isotype control BD Biosciences Cat# 553485 RRID:AB_479649
Anti-mouse Fc Block BD Biosciences Cat# 553142 RRID:AB_394657
Anti-mouse B220 BUV737 BD Biosciences Cat# 612838 RRID:AB_2738813
Anti-mouse CD3 PerCP-Cy5.5 BD Biosciences Cat# 551163 RRID:AB_394082
Anti-mouse CD138 BV650 BD Biosciences Cat# 564068 RRID:AB_2738574
Anti-mouse CD38 Alexa Fluor 700 Thermo Fisher Scientific Cat# 56-0381-82 RRID:AB_657740
Anti-mouse GL7 ef450 Thermo Fisher Scientific Cat# 48-5902-82 RRID:AB_10870775
Anti-mouse IgM BV786 BD Biosciences Cat# 743328 RRID:AB_2741429
Anti-mouse IgD BUV395 BD Biosciences Cat# 565988 RRID:AB_2737433
Anti-mouse CD73 PE-Cy7 Thermo Fisher Scientific Cat# 25-0731-82 RRID:AB_10853348
Anti-mouse CD80 BV605 BD Biosciences Cat# 563052 RRID:AB_273795

Biological samples

BALB/c mice Jackson Laboratory Cat# 000651

Chemicals, peptides, and recombinant proteins

AddaVax adjuvant InvivoGen Cat# vac-adx-10
ABTS ThermoFisher Cat# 37615
TMB SeraCare Cat# 5120-0083
EZ-Link Sulfo-NHS-LC Biotinylation Kit ThermoFisher Cat# 21435
Streptavidin-APC Agilent Cat# PJ27S-1
Streptavidin-PE Agilent Cat# PJRS25-1
Anti-PE Micro-Beads Miltenyi Biotec Cat# 130-048-801
Anti-APC Micro-Beads Miltenyi Biotec Cat# 130-090-855
DyLight 755 Antibody Labeling Kit ThermoFisher Cat# 84538
Alexa Fluor 647 Protein Labeling Kit ThermoFisher Cat# A20173

Experimental models: Cell lines

Expi293F ThermoFisher Cat# A14527
MDCK-SIAT-PB1 Creanga et al.98 N/A

Recombinant DNA

See Table S1 for amino acid sequences.

Software and algorithms

UCSF ChimeraX Goddard et al.99 https://www.rbvi.ucsf.edu/chimerax/
Prism GraphPad https://www.graphpad.com/scientific-software/prism/
FlowJo v10 FlowJo https://www.flowjo.com
RosettaScripts “sugarcoat” code Adolf-Bryfogle et al.1 N/A

Other

EM supplies 300 mesh grids Ted Pella Cat# 01843-F
Filter paper Cytiva Cat# 1004047
Uranyl formate SPI Chem Cat# 02545-AA
Superdex 200 Increase SEC column Cytiva Cat# 28-9909-44
Superose 6 Increase SEC column Cytiva Cat# 29091596
Talon resin TaKaRa Cat# 635652
Excel resin Cytiva Cat# 17371203
Isoflurane USP Patterson Cat# 07-893-1389
EndoSafe LAL Test Cartridges Charles River Labs Cat# PTS20005F
Lemo21(DE3) New England BioLabs Cat# C2528J
Isopropyl-B-D-thiogalactoside (IPTG) Sigma-Aldrich Cat# I6758
Kanamycin Sulfate Sigma-Aldrich Cat# K1876
HisTrap FF Cytiva Cat#17525501