Antibodies |
|
Horse anti-mouse HRP |
Cell Signaling Technology |
Cat# 7076S RRID:AB_330924 |
Mouse anti-myc mAb |
Cell Signaling Technology |
Cat# 2276S RRID:AB_331783 |
Goat anti-mouse IgG+IgM |
Jackson ImmunoResearch |
Cat# 115-005-068 RRID:AB_2338453 |
Mouse IgG1 lambda isotype control |
BD Biosciences |
Cat# 553485 RRID:AB_479649 |
Anti-mouse Fc Block |
BD Biosciences |
Cat# 553142 RRID:AB_394657
|
Anti-mouse B220 BUV737 |
BD Biosciences |
Cat# 612838 RRID:AB_2738813
|
Anti-mouse CD3 PerCP-Cy5.5 |
BD Biosciences |
Cat# 551163 RRID:AB_394082
|
Anti-mouse CD138 BV650 |
BD Biosciences |
Cat# 564068 RRID:AB_2738574
|
Anti-mouse CD38 Alexa Fluor 700 |
Thermo Fisher Scientific |
Cat# 56-0381-82 RRID:AB_657740
|
Anti-mouse GL7 ef450 |
Thermo Fisher Scientific |
Cat# 48-5902-82 RRID:AB_10870775
|
Anti-mouse IgM BV786 |
BD Biosciences |
Cat# 743328 RRID:AB_2741429
|
Anti-mouse IgD BUV395 |
BD Biosciences |
Cat# 565988 RRID:AB_2737433
|
Anti-mouse CD73 PE-Cy7 |
Thermo Fisher Scientific |
Cat# 25-0731-82 RRID:AB_10853348
|
Anti-mouse CD80 BV605 |
BD Biosciences |
Cat# 563052 RRID:AB_273795
|
|
Biological samples |
|
BALB/c mice |
Jackson Laboratory |
Cat# 000651 |
|
Chemicals, peptides, and recombinant proteins |
|
AddaVax adjuvant |
InvivoGen |
Cat# vac-adx-10 |
ABTS |
ThermoFisher |
Cat# 37615 |
TMB |
SeraCare |
Cat# 5120-0083 |
EZ-Link Sulfo-NHS-LC Biotinylation Kit |
ThermoFisher |
Cat# 21435 |
Streptavidin-APC |
Agilent |
Cat# PJ27S-1 |
Streptavidin-PE |
Agilent |
Cat# PJRS25-1 |
Anti-PE Micro-Beads |
Miltenyi Biotec |
Cat# 130-048-801 |
Anti-APC Micro-Beads |
Miltenyi Biotec |
Cat# 130-090-855 |
DyLight 755 Antibody Labeling Kit |
ThermoFisher |
Cat# 84538 |
Alexa Fluor 647 Protein Labeling Kit |
ThermoFisher |
Cat# A20173 |
|
Experimental models: Cell lines |
|
Expi293F |
ThermoFisher |
Cat# A14527 |
MDCK-SIAT-PB1 |
Creanga et al.98
|
N/A |
|
Recombinant DNA |
|
See Table S1 for amino acid sequences. |
|
|
|
Software and algorithms |
|
UCSF ChimeraX |
Goddard et al.99
|
https://www.rbvi.ucsf.edu/chimerax/ |
Prism |
GraphPad |
https://www.graphpad.com/scientific-software/prism/ |
FlowJo v10 |
FlowJo |
https://www.flowjo.com |
RosettaScripts “sugarcoat” code |
Adolf-Bryfogle et al.1
|
N/A |
|
Other |
|
EM supplies 300 mesh grids |
Ted Pella |
Cat# 01843-F |
Filter paper |
Cytiva |
Cat# 1004047 |
Uranyl formate |
SPI Chem |
Cat# 02545-AA |
Superdex 200 Increase SEC column |
Cytiva |
Cat# 28-9909-44 |
Superose 6 Increase SEC column |
Cytiva |
Cat# 29091596 |
Talon resin |
TaKaRa |
Cat# 635652 |
Excel resin |
Cytiva |
Cat# 17371203 |
Isoflurane |
USP Patterson |
Cat# 07-893-1389 |
EndoSafe LAL Test Cartridges |
Charles River Labs |
Cat# PTS20005F |
Lemo21(DE3) |
New England BioLabs |
Cat# C2528J |
Isopropyl-B-D-thiogalactoside (IPTG) |
Sigma-Aldrich |
Cat# I6758 |
Kanamycin Sulfate |
Sigma-Aldrich |
Cat# K1876 |
HisTrap FF |
Cytiva |
Cat#17525501 |