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. 2022 Oct 4;25(11):105275. doi: 10.1016/j.isci.2022.105275

Figure 4.

Figure 4

Construction and identification of meningioma cell line with NF2 c.770-784 heterozygous deletion

(A) Schematic diagram of guide RNA and pMD19-NF2-mut construction using the CRISPR/Cas9 system.

(B) Partial sequencing chromatograms of NF2+/− from gene-edited cells.

(C) NF2 mRNA identification of gene-edited cells.

(D) The sequence chromatograms of partial cDNA sequences of WT-IOMM and NF2+/−IOMM.

(E) The schematic diagram of pSPL3-NF2-mut plasmids.

(F) Partial sequence chromatograms and gel electrophoresis of RT-PCR products in pSPL3-NF2 (WT or mut) transfected cells.

(G) The schematic diagram of splicing both in WT-IOMM and NF2+/−IOMM.