Figure 5.

NF2 mutant promoted cell proliferation through inactivating Hippo pathway

(A, A′, A″, A‴) Western blotting and quantification of NF2, LATS1/2, YAP and TEAD. GAPDH is shown as a loading control.

(B) The immunofluorescence pattern of RFP-YAP1 and GFP-NF2 in HEK293T cells, which were transfected with exogenous GFP-NF2 (WT or mut) and RFP-YAP1. Scale bar, 50μm.

(C) Western blotting of YAP subcellular fractionation. Vinculin and Lamin B are shown as loading control of cytoplasm and nucleus, respectively.

(D and E) Relative expression level of CTGF and CYR61 by qPCR.

(F) Observation of WT-IOMM and NF2^+/−IOMM meningioma cells under light microscope.

(G) Growth curve of WT-IOMM and NF2^+/−IOMM meningioma cells. Error bars, SEM of three micrographs.

(H) Doubling time of WT-IOMM and NF2^+/−IOMM meningioma cells.

(I, I′, I″) Observation, quantification on rate of clone formation and clone size of WT-IOMM and NF2^+/−IOMM meningioma cells.

(J, J′) Observation and quantification on in vitro wound-healing assay of WT-IOMM and NF2^+/−IOMM meningioma cells. Error bars, SEM of three independent experiments. Data are represented as mean ± SEM ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, Student’s t test.