REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal Anti-GAPDH	Abcam	Cat# ab181602; RRID: AB_2630358
Rabbit polyclonal Anti-flag	Protein tech	Cat# 20543-1-AP; RRID:AB_11232216
Mouse monoclonal Anti-HA	Sino Biological	Cat# 100028-MM10; RRID:AB_2860039
Mouse monoclonal Anti-GST	Protein tech	Cat# HRP-66001; RRID:AB_2883833
Rabbit monoclonal Anti-YAP	Cell Signaling Technology	Cat# 14074; RRID:AB_2650491
Rabbit polyclonal Anti-phospho-YAP(S127)	Cell Signaling Technology	Cat# 4911S; RRID:AB_2218913
Rabbit monoclonal Anti-LATS1	Cell Signaling Technology	Cat# 3477S; RRID:AB_2133513
Rabbit monoclonal Anti-LATS1(T1027)	Cell Signaling Technology	Cat# 8654S; RRID:AB_10971635
Rabbit monoclonal Anti-NF2	Abcam	Cat# ab109244; RRID:AB_10866511
Rabbit polyclonal Anti-TEAD1	Affinity	Cat# DF3141; RRID:AB_2835518
Rabbit monoclonal Anti-Ki67	Cell Signaling Technology	Cat# 9027; RRID:AB_2636984
Mouse monoclonal Anti-CD31	Cell Signaling Technology	Cat# 3528; RRID:AB_2160882
Rabbit monoclonal Anti-Vinculin	Cell Signaling Technology	Cat# 13901T; RRID:AB_2728768
Rabbit monoclonal Anti-Lamin B	Cell Signaling Technology	Cat# 13435S; RRID:AB_2737428
House polyclonal Anti-mouse IgG-HRP	Cell Signaling Technology	Cat# 7076; RRID:AB_330924
Goat polyclonal Anti-rabbit IgG-HRP	Cell Signaling Technology	Cat# 7074; RRID:AB_2099233
Chemicals
Trizol	Invitrogen	Cat # 15596026
NucleoBond® CB 100	TaKaRa	Cat# 740508
PrimeSTAR® Max DNA Polymerase	TaKaRa	Cat# R045A
SuperScript III First-Strand Synthesis SuperMix	Invitrogen	Cat# 18080400
RNase H	Thermo Scientific	Cat# EN0201
Lipofectamine® 3000 Transfection Kit	Invitrogen	Cat# L3000075
CellMask™ Deep Red Plasma membrane Stain	Invitrogen	Cat# C10046
DAPI	Thermo Scientific	Cat# 62248
1,2-Dipalmitoyl-sn-glycero-3-phosphate	MedChemExpress	Cat# HY-113437
Subcellular Protein Fractionation Kit	Thermo Scientific	Cat# 78840
ClonExpress ® MultiS One Step Cloning Kit	Vazyme	Cat# C113
SuperReal PreMix Plus (SYBR Green)	TIANGEN	Cat# FP205
Dulbecco’s Modified Eagle Medium	Gibco	Cat# 11965118
Fetal bovine serum	Gibco	Cat# 16000-044
Bacterial and virus strains
TOP10 Chemically Competent Cell	YEASEN	Cat# 11801ES80
Rosetta Competent Cell	TIANGEN	Cat# CB108
Experimental models: cell lines
HEK293T cell lines	American Type Culture Collection	Cat# CRL-3216;RRID:CVCL_0063
IOMM-Lee cell lines	American Type Culture Collection	Cat# CRL-3370; RRID:CVCL_5779
Experimental models: Organisms/strains
BALB/c nude mice	Shanghai Laboratory Animal Center	N/A
Oligonucleotides
NF2-Exon1 Forward: CTGCCACTGTAGCCACTCAA	This paper	N/A
NF2-Exon1 Reverse: AGCCTGACACTGAAATCCTGAA	This paper	N/A
NF2-Exon2 Forward: TGTCCAAGGGATGTATTGTGTATG	This paper	N/A
NF2-Exon2 Reverse: CTGCATGACTTAATTCAGGAATGTG	This paper	N/A
NF2-Exon3 Forward: CTTAATCCTAGCTCTGCTCACTTC	This paper	N/A
NF2-Exon3 Reverse: ATTCTTCACCATCACACCTTCAC	This paper	N/A
NF2-Exon4 Forward: ACTGAACTCTCCACCTGTCTG	This paper	N/A
NF2-Exon4 Reverse: GTCCACAAGTCCCATAACATCTC	This paper	N/A
NF2-Exon5 Forward: GACTTGTCATCGGAATTGAATTGC	This paper	N/A
NF2-Exon5 Reverse: CCCACACTGTTTACTGGAGATTAC	This paper	N/A
NF2-Exon6 Forward: TGAGTTGCTCCTCCAATAGAATG	This paper	N/A
NF2-Exon6 Reverse: CTTGACAGGCAGTACACATACC	This paper	N/A
NF2-Exon7 Forward: AAGGGCAAGGTGGGAAACTAA	This paper	N/A
NF2-Exon7 Reverse: AGCAGAACCAGGTCCAAGTC	This paper	N/A
NF2-Exon8 Forward: CAGAAGTCACAAGAATGGAACAGT	This paper	N/A
NF2-Exon8 Reverse: ATCAACAACCACACCCTCAAAG	This paper	N/A
NF2-Exon9 Forward: CCAGGACAAGGCATAACTTCAT	This paper	N/A
NF2-Exon9 Reverse: CGCCAAGTGAGATACCATTCTATA	This paper	N/A
NF2-Exon10 Forward: TCCAGAGCTGACGGCACTA	This paper	N/A
NF2-Exon10 Reverse: GTAGGCATCGGCAAATGAAGAT	This paper	N/A
NF2-Exon11 Forward: TGTTCATTCACCACTAGACTGT	This paper	N/A
NF2-Exon11 Reverse: AATCTGCTTCTGCCAACACA	This paper	N/A
NF2-Exon12 Forward: GGCTTGTCATTTCTTGTCAGAGG	This paper	N/A
NF2-Exon12 Reverse: AAGAGACACAAGTATGACCAGAGA	This paper	N/A
NF2-Exon13 Forward: TGTTGTCCTTGCTTGGAAGTTC	This paper	N/A
NF2-Exon13 Reverse: GGCAGACCAGGATGTTCTACC	This paper	N/A
NF2-Exon14 Forward: GGGAGTGAAGTGGAGGGATG	This paper	N/A
NF2-Exon14 Reverse: TGAATGCTGATCTGTTGTCCTT	This paper	N/A
NF2-Exon15 Forward: CCTAGATCGCACACCAAGCA	This paper	N/A
NF2-Exon15 Reverse: CTCAACTTCCATCCAGCCAATG	This paper	N/A
NF2-Exon16 Forward: CCAGGAACAGTCTCACCATT	This paper	N/A
NF2-Exon16 Reverse: GGATAACGACCACAAGTAGGAT	This paper	N/A
NF2-Exon17a Forward: GACAGCCAACTTCTTGAGCAT	This paper	N/A
NF2-Exon17a Reverse: GGAGGGAAGGTGAAGCAGAT	This paper	N/A
NF2-Exon17b Forward: CATAGACGCTCCTTCCTGTGT	This paper	N/A
NF2-Exon17b Reverse: CAACATCCATACTTACGCTCTAGG	This paper	N/A
NF2-Exon17c Forward: ATCCTGCCTTCTTCCTACTATCA	This paper	N/A
NF2-Exon17c Reverse: GAGGTGCGTGCCTGTAATC	This paper	N/A
NF2-Exon17d Forward: TGGAGTGCAATGGCGTGAT	This paper	N/A
NF2-Exon17d Reverse: GCAGTGAGAGACAGCAGAGG	This paper	N/A
NF2-mut-test Forward: GACCCTGAGAACAGACTGAC	This paper	N/A
NF2-mut-test Reverse: ACCTCCTTGTCACTGTACGA	This paper	N/A
CRISPR-NF2-del-test Forward: TCAGCTGGCGCTTACAGTAG	This paper	N/A
CRISPR-NF2-del-test Reverse: GGGAAAGATCTGCTGGACCC	This paper	N/A
pSPL3-test Forward: TCACCTGGACAACCTCAAAG	This paper	N/A
pSPL3-test Reverse: TGAGGAGTGAATTGGTCGAA	This paper	N/A
NF2-WT Forward: ATGGCCGGGGCCATCGCTTC	This paper	N/A
NF2-WT Reverse: GAGCTCTTCAAAGAAGGCCAC	This paper	N/A
NF2-mut Forward: CGAAACATCTCGTACAGTGAC	This paper	N/A
NF2-mut Reverse: CGAGATGTTTCGGAA GGAGATCTTGGGGGTCAG	This paper	N/A
NF2-p257del Forward: TGGAATGAAATCCGAAACATC	This paper	N/A
NF2-p257del Reverse: GATTTCATTCCAGAA GGAGATCTTGGGGGTCAG	This paper	N/A
NF2-p258del Forward: AATGAAATCCGAAACATCTCG	This paper	N/A
NF2-p258del Reverse: TCGGATTTCATTCGGGA AGGAGATCTTGGGGGT	This paper	N/A
NF2-p259del Forward: GAAATCCGAAACATCTCGTAC	This paper	N/A
NF2-p259del Reverse: GTTTCGGATTTCCCA CGGGAAGGAGATCTTGGG	This paper	N/A
NF2-p260del Forward: ATCCGAAACATCTCGTACAGT	This paper	N/A
NF2-p260del Reverse: GATGTTTCGGATATTCCA CGGGAAGGAGATCTT	This paper	N/A
NF2-p261del Forward: CGAAACATCTCGTACAGTGAC	This paper	N/A
NF2-p261del Reverse: CGAGATGTTTCGTTCATTC CACGGGAAGGAGAT	This paper	N/A
NF2-skip Forward: GAACTACTTTGCAATCCGGT TTACTATTAAACCACTGG	This paper	N/A
NF2-skip Reverse: CCAGTGGTTTAATAGTAAAC CGGATTGCAAAGTAGTTC	This paper	N/A
YAP Forward: GAGGCAGAAGCCATGGATC	This paper	N/A
YAP Reverse: AATTCAGTCTGCCTGAGGGC	This paper	N/A
LATS1 Forward: ATGAAGAGGAGTGAAAAGCC	This paper	N/A
LATS1 Reverse: GTTAAACATATACTAGATCGC	This paper	N/A
pGEX-4T2-NF2 Forward: ATCTGGTTCCGCGTGGATC CATGGCCGGGGCCATCGCTTC	This paper	N/A
pGEX-4T2-NF2 Reverse: GTCAGTCACGATGCGGCCG CGAGCTCTTCAAAGAAGGCCA	This paper	N/A
pX459-NF2-del Forward: CACCGTCGGATTTCATTCCACGGGA	This paper	N/A
pX459-NF2-del Reverse: AAACTCCCGTGGAATGAAATCCGAC	This paper	N/A
pSPL3-NF2 Forward: ACGGGATCACCAGAATTC AGCCAGTGACAGAAGCCCTCAG	This paper	N/A
pSPL3-NF2 Reverse: ACCAGATATCTGGGATCC CACACCCTCAAAGCCTGGGAAT	This paper	N/A
EGFP-NF2 Forward: CGAGCTCAAGCTTCGAATT ATGGCCGGGGCCATCGCTTC	This paper	N/A
EGFP-NF2 Reverse: GACCGGCCGGTGGATCGC GAGCTCTTCAAAGAAGGCCAC	This paper	N/A
RFP-LATS1 Forward: GATCTCGAGCTCAAGCTT CGATGAAGAGGAGTGAAAAGC	This paper	N/A
RFP-LATS1 Reverse: CTCGGAGGAGGCCATGG ATCCAACATATACTAGATCGCG	This paper	N/A
3×HA-NF2 Forward: ATTGAATTCCCCGGGGAT CCATGGCCGGGGCCATCGCTTC	This paper	N/A
3×HA-NF2 Reverse: TATGGGTAGTCGACTC TAGAGAGCTCTTCAAAGAAGGCCAC	This paper	N/A
3×Flag-LATS1 Forward: AAGCTTGCGGCCGCG AATTCATGAAGAGGAGTGAAAAGCC	This paper	N/A
3×Flag-LATS1 Reverse: TTGTAGTCAGCCCGGGAT CCAACATATACTAGATCGCGAT	This paper	N/A
RFP-YAP Forward: CGAGCTCAAGCTTCGAATTCA TGGATCCCGGGCAGCAGCC	This paper	N/A
RFP-YAP Reverse: CGGAGGAGGCCATGGAT CCTAACCATGTAAGAAAGCTTTC	This paper	N/A
3×Flag-YAP Forward: GCTTGCGGCCGCGAATTC ATGGATCCCGGGCAGCAGCC	This paper	N/A
3×Flag-YAP Reverse: GTAGTCAGCCCGGGATCC TAACCATGTAAGAAAGCTTTC	This paper	N/A
GAPDH Forward: CAGGGCTGCTTTTAACTCTGGTA	This paper	N/A
GAPDH Reverse: CATGGGTGGAATCATATTGGAAC	This paper	N/A
CTGF-qPCR Forward: CCAATGACAACGCCTCCTG	This paper	N/A
CTGF-qPCR Reverse: TGGTGCAGCCAGAAAGCTC	This paper	N/A
CYR61-qPCR Forward: AGCCTCGCATCCTATACAACC	This paper	N/A
CYR61-qPCR Reverse: TTCTTTCACAAGGCGGCACTC	This paper	N/A
Recombinant DNA
pEGFP-NF2-WT	This paper	N/A
pEGFP-NF2-mut	This paper	N/A
pEGFP-NF2-p.257del	This paper	N/A
pEGFP-NF2-p.258del	This paper	N/A
pEGFP-NF2-p.259del	This paper	N/A
pEGFP-NF2-p.260del	This paper	N/A
pEGFP-NF2-p.261del	This paper	N/A
pGEX-NF2-WT	This paper	N/A
pGEX-4T2-NF2-mut	This paper	N/A
pGEX-4T2-NF2-p.257del	This paper	N/A
pGEX-4T2-NF2-p.258del	This paper	N/A
pGEX-4T2-NF2-p.259del	This paper	N/A
pGEX-4T2-NF2-p.260del	This paper	N/A
pGEX-4T2-NF2-p.261del	This paper	N/A
pRFP-LATS1	This paper	N/A
pRFP-YAP1	This paper	N/A
p3×HA-NF2-WT	This paper	N/A
p3×HA-NF2-mut	This paper	N/A
p3×Flag-LATS1	This paper	N/A
p3×Flag-YAP1	This paper	N/A
pX459-NF2-mut	This paper	N/A
pMD19-NF2-mut	This paper	N/A
Software and algorithms
Snapgene	Snepgene	N/A
ImageJ	ImageJ	N/A
GraphPad Prism 8	GraphPad	N/A
Adobe Photoshop	Adobe Photoshop	N/A