Figure 2.

The post-dissection liver metabolome is rapidly remodeled by hypoxia. (A) Schematic depicting the dependence of the NADH oxidation and O₂ reduction activities of the electron transport chain (ETC) on the ATP synthesis activity of the ATP synthase/Complex V (V). (B–C) Line graphs showing the NAD⁺ and NADH (B) and AMP, ADP, and ATP (C) metabolite fold changes by delayed freezing time-point compared to time zero. Data are presented as the mean ± SEM. P-values were calculated by one-way ANOVA with the post-hoc Holm-Sidak multiple comparison test (n = 6 biological replicates). (D) Representative Western blot of liver tissue p-AMPK (Thr172), AMPK, and Hsp90 by delayed freezing time-point and a line graph showing p-AMPK/AMPK quantification. Quantified densiometric data are presented as the mean ± SEM. P-values were calculated by one-way ANOVA with the post-hoc Holm-Sidak multiple comparison test (n = 6 biological replicates). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; Related to Figure S2 and Table S1.