Figure 5.

Delayed post-dissection freezing of heart and skeletal muscle results in a shift to hypoxic metabolism. (A) Volcano plot showing the interaction between the heart metabolite fold changes and associated p-values at 2-minutes delayed freezing compared to time zero. The purine degradation pathway metabolite data points are labeled and colored blue. The -log transformed p-values were calculated by Student's t-test. The number of significant differences for each time-point are listed in the panel (n = 5–6 biological replicates). (B) Bar graph showing the heart acyl-carnitine fold changes at 2-minutes delayed freezing compared to time zero. Data are presented as the mean ± SEM. P-values were calculated by Student's t-test (n = 5–6 biological replicates). (C) Volcano plot showing the interaction between the skeletal muscle metabolite fold changes and associated p-values at 1-minute delayed freezing compared to time zero. α-Ketoglutarate and succinate data points are labeled and colored blue. The -log transformed p-values were calculated by Student's t-test. The number of significant differences for each time-point are listed in the panel (n = 5–6 biological replicates). (D) Bar graph showing the skeletal muscle α-ketoglutarate and succinate fold changes at 1-minute delayed freezing compared to time zero. Data are presented as the mean ± SEM. P-values were calculated by Student's t-test (n = 5–6 biological replicates). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; Related to Figure S4 and Tables S3 and S4. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)