Figure 6.

Delayed liver freezing leads to both false negative and false positive between-genotype metabolite level differences. (A) Heatmap depicting the z-scores of the MPC-LivKO/WT metabolite-level ratios by delayed freezing time-point compared to the MPC-LivKO/WT ratio at time zero. The heatmap is not time-scaled (n = 4–5 biological replicates). (B–E) Line graphs showing MPC-LivKO (blue) and WT (gray) pyruvate (B), α-ketoglutarate (C), acetoacetyl-CoA (D) and propionate (E) fold changes by delayed freezing time-point. Data are presented as the mean ± SEM. P-values between genotypes were calculated using the Student's t-test (n = 4–5 biological replicates). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; Related to Figure S5 and Tables S5 and S6. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)