FIGURE 2.
CCK8 assay was performed to determine the effects of 17 β-estradiol on cell proliferation in hUSLFs. The fibroblast cell growth rate of POP was obviously lower than that of Control (A). Physiological 17 β-estradiol concentration (10−10 M(B) and 10−9 M(C) did not obviously influence the cell proliferation for both groups in the entire culture process. Estrogen treatment increased the proliferation of both control and POP fibroblast cells at 24 h, but the increase was not statistically significant (D) and did not continue in 48 h (E) and 72 h (F). Values are expressed as the mean ± standard deviation of three independent experiments. ***p < 0.001 POP vs Control.