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. 2022 Oct 10;13:980843. doi: 10.3389/fphys.2022.980843

FIGURE 3.

FIGURE 3

Mechanical stress induced apoptosis of hUSLFs occurs in a loading-dependent manner. (A) Representative dot plots of cell apoptosis by flow cytometry analysis after Annexin V/7-AAD dual staining. The apoptotic rate was determined as the percentage of 7-AAD negative and Annexin V positive (Q3). Cells that are already dead are both Annexin V and 7-AAD positive (Q2). (A,D) control; (B,E) 10% strain, 0.5 Hz; (C,F) 20% strain, 0.5 Hz. (G) Quantified apoptotic rates of hUSLFs treated with various intensities of mechanical strains for 24 h. (H) Quantified dead rates of hUSLFs treated with various intensities of mechanical strains for 24 h. Values are expressed as the mean ± standard deviation of three independent experiments. *p < 0.05; ***p < 0.001 vs. control.