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. 2022 Oct 10;13:1036641. doi: 10.3389/fgene.2022.1036641

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Copyright © 2022 Hu, Pan, Shi, Qiu, Wang, Murawala, Liu, Xing, Tanaka and Fei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphology and patterning of limb regenerates in Pax7 mutants. (A) Bright-field images of the limbs at indicated dpa, from 3-month-old controls and Pax7 mutants. Scale bars, 1 mm. (B) Alcian blue and Alizarin red-stained fully regenerated (left panels) and un-operated (right panels) forelimbs from controls (upper panels) and Pax7 mutants (lower panels). Amputations were carried out on 6-month-old animals, then allowed them to regenerate for 10-months for analysis. Hand part of each stained forelimb is presented at higher magnification, in which the red and yellow lines outline the phalange, metacarpal bones and carpal bones, respectively. Scale bars, 2 mm. (C) The elbow joint of regenerated (left panels) and un-operated (right panels) forelimbs from controls (upper panels) and Pax7 mutants (lower panels). Scale bars, 500 μm.