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. 2022 Oct 10;13:1036641. doi: 10.3389/fgene.2022.1036641

FIGURE 2.

FIGURE 2

Macrophage dynamics in limb regeneration of Pax7 mutants. (A) Immunofluorescence images of IBA1 (green) and DAPI (white) in limb longitudinal-sections, at 0, 4, 9 and 16 dpa, from control and Pax7 mutant animals. Boxed regions are shown at higher magnification as separated channels. Red dashed lines, amputation planes. Scale bars, 200 μm. (B) and (C) Quantification of the absolute number (B) and the percentage (C) of IBA1 positive cells, within the areas of the newly formed blastema combined 500 μm zone proximal to the amputation plane, at 0, 4, 9 and 16 dpa, from controls (n = 4) and Pax7 mutants (n = 4). Data are presented as mean ± sem, *p < 0.05, **p < 0.01.