TABLE 1.

Tests for EBV-specific antibodies

Test	Manufacturer	Antibody detection	Test principle, antigen source, and mode of detection
CAPTIA Select VCA-M/G/EBNA	Centocor Inc.	VCA, EBNA	VCA IgM, μ-capture EIA; VCA and EBNA IgG, indirect EIA; conjugate, biotinylated synthetic peptide from the p18 protein and peroxidase-labeled streptavidin; qualitative detection
Enzygnost anti-EBV/IgM and IgG	Behringwerke	Combination of VCA, EA, and EBNA	Indirect EIA; antigen, combination of EBV-specific epitopes; detection of IgM and IgG antibodies, respectively, with peroxidase-labeled anti-human IgM and IgG; control antigen is included; qualitative and quantitative detection
Vironostika EBV VCA IgM/IgG/EBNA ELISA	Organon Teknika	VCA, EBNA	VCA IgM, μ-capture EIA; conjugate, monoclonal anti-VCA p18 labeled with peroxidase; synthetic VCA p18 peptide antigen is added to the conjugate diluent; VCA and EBNA IgG, indirect EIA; coating with synthetic p18 peptide antigen; qualitative and semiquantitative detection
SEROFLUOR IFA and EBV Combi-Test	Institute Virion Ltd.	VCA (SEROFLUOR), VCA, EA, EBNA (EBV Combi-Test)	For SEROFLUOR, indirect IFA; antigen source, P3HR-1 cells, for EBV Combi-Test, anti-complement IF antigen source, a β-lymphocyte cell line which expresses VCA, EA, and EBNA; differentiation of VCA and EBNA antibodies is based on different reading pattern; qualitative detection
Anti-EBV recombinant IgM and IgG EA/EBNA IgG ELISA	Biotest Diagnostics	EA, EBNA	Indirect EIA with recombinant antigen; EA p54 and p138 for EA IgM/IgG and EBNA-1 p-172; qualitative and semiquantitative detection
EBV IgM/IgG/EBNA ELISA	Gull Laboratories	VCA, EBNA	Indirect EIA; antigen source for VCA IgM/IgG, gp125 cultivated in P3HR-1 cells; for EBNA IgG, recombinant antigen corresponding to the C-terminal part of the EBNA-1 gene product (without IR3); qualitative detection