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. 2022 Jun 14;22(6):872–881. doi: 10.17305/bjbms.2022.7348

FIGURE 2.

FIGURE 2

The effect of IRAK1/4-inh on the DSS-induced TLR4/NF-κB pathway in colon tissue. (A) Typical Western blotting images of TLR4, IRAK1, IRAK4, cytoplasmic p65, nuclear p65, IκBα, and p-IκBα. β-actin was the standard reference for the gray analysis. (B) TLR4, (C) IRAK1, (D) IRAK4, (E) p65 (cytoplasmic), (F) p65 (nuclear), and (G) p-IκBα/IκBα were protein relative expression levels. Also, the effect of IRAK1/4-inh on IRAK1 (H) and IRAK4 (I) mRNA levels is shown. Results are displayed as the mean ± SD (n = 3-8). *p < 0.05, **p < 0.01 versus Control group, #p < 0.05, ##p < 0.01 versus DSS group, IRAK1/4-inh: Interleukin-1 receptor-associated kinase ¼ inhibitor.