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. 2022 Aug 23;45(10):718–728. doi: 10.14348/molcells.2022.0037

Fig. 5. UBE4B is involved in p53 stabilization upon SF3B4 depletion in A549 cells.

Fig. 5

(A) Interaction between SF3B4 and UBE4B was verified by immunoprecipitation with anti-UBE4B antibody followed by western blotting with anti-SF3B4. IP, immunoprecipitation; Ab, antibody. (B) Effect of SF3B4 knockdown on the expression of UBE4B as determined by western blotting (left, representative result from three independent experiments) and qRT-PCR (right) after exposure to the indicated siRNA for 48 h. (C) Under SF3B4 depletion, the degradation rate of UBE4B was determined by CHX (10 μg/ml) chase assay (left). UBE4B levels are represented as mean ± SD from three independent experiments (right panels in B and C). *P < 0.05 and **P < 0.01 with respect to control values.