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Concentration of antibodies to muscarinic cholinergic receptor 4 (anti-M4): (A) Healthy control (donors) and ME/CFS patients. (B) Regarding women and men of both groups. (C) Regarding ME/CFS severity; numbers above graphs show p-value, determined by Mann–Whitney test (A) or Kruskal–Wallis test with respective post-test (B, C). (D, E) ROC analysis showing the potential of anti-M4 as a discriminating marker for ME/CFS.
