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. 2022 Oct 10;13:994025. doi: 10.3389/fphar.2022.994025

FIGURE 2.

FIGURE 2

O. syriacum inhibits cellular proliferation of MDA-MB-231 breast cancer cells. (A) MDA-MB-231 cells were treated or not with the indicated concentrations of O. syriacum ethanolic extract (OSEE) for 24, 48, and 72 h. For vehicle control, a concnetration of ethanol equivalent to that present in OSEE was used. Cell viability was monitored using the metabolism-based MTT assay, as described in Materials and Methods. (B) MDA-MB-231 cells were incubated for 24 h with and without the indicated concentrations of OSEE. Cells were then lysed, and protein lysates were subject to Western blotting with a Ki67 antibody. Data represent the mean ± SEM of three independent experiments (n = 3) carried out in triplicate and expressed as a percentage of the corresponding control cells. Statistical analysis was performed using one-way ANOVA followed by LSD post-hoc test (*p < 0.05, **p < 0.005, ***p < 0.001).