Skip to main content
. 2022 Oct 10;13:994025. doi: 10.3389/fphar.2022.994025

FIGURE 9.

FIGURE 9

O. syriacum reduces the invasive potential of MDA-MB-231 cells. (A) MDA-MB-231 cells were incubated overnight with and without the indicated concentrations of OSEE inBoyden chamber trans-well inserts pre-coated with Matrigel as described in Materials and Methods. Cells that invaded the Matrigel were stained with DAPI, imaged, and then counted and analyzed. Values represent the fold change in migration of the ethanol-treated control. The experiment was repeated three times (n = 3) and data represent the mean ± SEM (**p < 0.005, ****p < 0.0001). (B) Cells were treated with and without 200 μg/ml OSEE at different time points and the phosphorylation of FAK was assessed at hose points by Western blotting, using total-FAK as loading control. The Western blot is representative of three independent experiments (n = 3). **p < 0.005, ****p < 0.0001.