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. 2022 Oct 10;12:1018285. doi: 10.3389/fonc.2022.1018285

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Copyright © 2022 Shen, Li, Zhang, Zhang, Wu, Da, Yang, Wang, Zhang, Qie, Zhao, Lin, Huang, Zhou and Hu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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circ_0067900 overexpression exerted a tumor suppressive effect in BCa cells. (A) Five circRNAs were randomly chosen to verify the circRNA-sequencing results in 20 pairs of samples by quantitative real-time PCR. (B) Lentiviral transduction efficiencies were evaluated using RT-qPCR in T24 and 253J-BV cells. (C) CCK-8 proliferation assay. (D) OE-circ_0067900 inhibited monoclonal formation ability of BCa cells. (E) Scratch assay of T24 and 253J-BV cells. Experiments were terminated after scratch for 24 h. (F) Migration and invasion were evaluated using a transwell assay without and with Matrigel, respectively. Data are showed as means ± SD; n = 3. SD, standard deviation; OE-, Overexpression; NC, negative control vector; CCK-8, cell counting kit-8; OD, optical density. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.