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. Author manuscript; available in PMC: 2022 Oct 24.
Published in final edited form as: Biotechnol Bioeng. 2020 Jun 10;117(9):2715–2727. doi: 10.1002/bit.27440

FIGURE 3.

FIGURE 3

Media development using design of experiment (DoE) methodology. 212 Media formulations were evaluated for autoinduction based on phosphate depletion, each comprising different “levels” of casamino acids, yeast extract, trace metals (TM mix), calcium sulfate (CaSO4), magnesium sulfate (MgSO4), iron(II) sulfate (FeSO4), ammonium sulfate ((NH4)2SO4), and citric acid. (a) Upper panel: GFP (green bars) and OD600 nm (gray bars) rank ordered plot for all media formulations. Standard deviations are from triplicate experiments. Lower panel: Nutrient concentration levels for all media (refer to Supporting Information Materials Section 4, for specific concentrations for each level). Strain DLF_R002 with plasmid pHCKan-yibDp-GFPuv was used for all experiments. (b) GFP fluorescence (green line), phosphate levels (black circles) and OD600 nm (black line) for strain DLF_R002 with plasmid pHCKan-yibDp-GFPuv in media #36 (autoinduction broth) media. Standard deviations (shaded regions) are from triplicate experiments. OD, optical density