TABLE 1.
Plasmids and strain used in this study
| Plasmid | Insert | Promoter | ori | Res | Addgene | Source |
|---|---|---|---|---|---|---|
| pSMART-HC-Kan | None | None | colE1 | Kan | NA | Lucigen |
| pLysS | T7 lysozyme | NA | p15a | Cm | NA | NEB |
| pHCKan-yibDp-GFPuv | GFPuv | yibDp | colE1 | Kan | 127078 | This study |
| pHCKan-yibDp-mCherry | 6xhis-mCherry | yibDp | colE1 | Kan | 127058 | This study |
| pETM6 | None | T7 (pET) | colE1 | Amp | 49795 | Jones et al. (2015) |
| pETM6-mCherry | mCherry | T7 (pET) | colE1 | Amp | 66534 | |
| pCDF | None | None | cloDF13 | Sm | 89596 | This study |
| pTCmc-yibDp-SBS-mCherry | SBS-mCherry | yibDp | p15a | Cm | 134598 | This study |
| pTKhc-yibDp-GFP-β20-cp6 | GFP-β20-cp6 | yibD | colE1 | Kan | 127060 | This study |
| pTKhc-yibDp-GFP-cp6 | GFP-cp6 | yibD | colE1 | Kan | 134938 | This study |
| pCDF-yibDp-matB | matB | yibDp | cloDF13 | Sm | 134597 | This study |
| pSMART-Ala1 | AlaDh(D196A/L197R) | yibDp | colE1 | Kan | 65814 | This study |
| pCDF-yibDp-mdlC-his | mdlC-6xhis | yibDp | cloDF13 | Sm | 134590 | This study |
| pHCKan-yibDp-GST | GST-6xHis | yibD | colE1 | Kan | 134592 | This study |
| pHCKan-yibDp-Nef | Nef | yibD | colE1 | Kan | 134593 | This study |
| pTKhc-yibDp-cimA3.7 | cimA3.7 | yibDp | colE1 | Kan | 134595 | This study |
| pHCKan-yibDp-CBD-hGLY | hGLYAT2 | yibD | colE1 | Kan | 134596 | This study |
| Strains used in this study | ||||||
| Strain | Genotype | Source | ||||
| BL21(DE3) | F– ompT gal dcm Lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) | NEB | ||||
| BWapldf | F-, λ-, Δ(araD-araB)567, lacZ4787(del)(::rrnB-3), rph-1, Δ(rhaD-rhaB)568, hsdR51, ΔackA-pta, ΔpoxB, ΔpflB, ΔldhA, ΔadhE |
Jian et al. (2010) | ||||
| DLF_R002 | BWapldf, ΔiclR, ΔarcA | This study | ||||
| DLF_R003 | DLF_R002, ΔompT::apmR | This study | ||||
Abbreviations: Amp, ampicillin; Cm, chloramphenicol; Kan, kanamycin; NEB, New England BioLabs; Res, resistance marker; Sm, spectinomycin.