Table1.

Differentiation factors and activation stimuli to derive BMDMs in vitro

Activated phenotype	Differentiation factor/colony proliferator (day 0, 3) (20–50 ng/ml)	Activation stimuli (day 6/7 +)	Reference
M1	M-CSF/GM-CSF	IFN-γ (20–50 ng/ml)	[37–39]
M1	M-CSF/GM-CSF	LPS (100 ng/ml)/IFN-γ (20 ng/ml)	[37, 38, 40]
M1	M-CSF/GM-CSF	IFNγ (50 ng/ml) + LPS (10 ng/ml)	[37, 38, 40]
M2c	M-CSF	TGF-β1 (20 ng/ml)	[41]
M2c	M-CSF	IL-10 (1 0 ng/ml)	[37, 38]
M2b	M-CSF	IgG (immobilized) + LPS (100 ng/ml)	[42]
M2a	M-CSF	IL-4/IL-4 + IL-13 (20 ng/ml/each)	[42–45]