. Author manuscript; available in PMC: 2022 Oct 24.

Published in final edited form as: Toxicology. 2021 May 10;457:152819. doi: 10.1016/j.tox.2021.152819

Table 1.

Lowest detectable depletion rate constant (k_dep) and corresponding lowest detectable in vitro intrinsic clearance rate (CL_int), determined by power analysis for each species and starting substrate concentration category

Species	Substrate concentration (μM)	Average RMSE^a of datasets	Lowest detectable k_dep (1/h)	Lowest detectable CL_int (µL/min/10⁶ hepatocytes)^b

Human	0.1	0.13	0.10	3.43
Human	1	0.09	0.08	2.57

Rat	0.1	0.11	0.09	2.93
Rat	1	0.19	0.15	5.07

Trout	0.1	0.21	0.17	2.80
Trout	1	0.25	0.20	3.38

RMSE = root mean square error

Calculated assuming 0.5 × 10⁶ hepatocytes/mL for human and rat, 1 × 10⁶ hepatocytes/mL for trout